-全讯担保网

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[(b.)-345.5(divergens)]tj /f12 1 tf 5.1465 0 td (and)tj /f11 1 tf 1.8457 0 td [(b.)-351.7(venatorum)]tj /f12 1 tf 5.4551 0 td [(,)-348.9(with)]tj /f11 1 tf 2.6898 0 td [(b.)-351.8(microti)]tj /f12 1 tf -22.8032 -1.3858 td [(being)-206.1(common)-198.5(in)-206.9(the)-199.8(south)-201.9(and)]tj /f11 1 tf 12.737 0 td [(b.)-206.9(venatorum)]tj /f12 1 tf 5.5118 0 td [(being)-199.8(prevalent)-207.8(in)-200.6(the)]tj -18.2488 -1.3921 td [(north)-332.1(\()]tj 0.30196 0.72157 0.33333 scn 2.9102 0 td [(qi)-330.1(et)-334(al.,)-332.1(2011)]tj 0.15294 0.15294 0.15294 scn 5.7512 0 td [(\).)-333.5(among)-328(the)-332.1(cases)-333.7(reported)-333.4(in)-326.6(china,)-336.5(a)-331.5(few)]tj -8.6614 -1.3858 td 0.0192 tc [(patients)-517.6(had)-512.8(a)-520.2(clear)-517.6(h)2.2(ist)6.2(o)-3.6(ry)-518.1(of)]tj 14.1165 0 td 0.0206 tc [(ti)3.4(ck)-517.2(bi)3.4(tes)-516.2(a)4.1(nd)-517.7(su)5.2(rgi)3.4(c)2(a)4.1(l)-516.8(bl)6(oo)4.1(d)]tj -14.1165 -1.3921 td 0 tc [(transfusions,)-307.2(but)-303.2(most)-296(patients)-303.6(had)-307.3(no)-300.7(clear)-296.5(infection)-309.2(mode.)]tj /f11 1 tf 1.5559 -1.3858 td (babesia)tj /f12 1 tf 3.2441 0 td [(infection)-214.7(of)-210.6(red)-211.8(blood)-211.9(cells)-212.6(results)-210.5(in)-213.2(lysis)-212.6(of)-210.6(the)-212.4(infected)]tj -4.8 -1.3921 td [(red)-381.9(blood)-381.9(cells,)-378.9(thus)-380.6(leading)-387.3(to)-375.9(hemolytic)-382.5(anemia.)-387.6(in)-379.5(addition)-382.8(to)]tj 0 -1.3858 td [(tick-mediated)-259.7(transmission,)]tj /f11 1 tf 11.3449 0 td (babesia)tj /f12 1 tf 3.2882 0 td [(can)-250.1(also)-256.9(be)-249.3(transmitted)-261.8(via)-249.7(the)]tj -14.6331 -1.3921 td [(t)-6.7(r)0(a)-11.6(n)0(s)-8.4(f)0(u)-7.9(s)-7.7(io)-8.5(n)-511(o)0(f)-512.9(i)0(n)-11.6(f)0(e)-11.1(c)0(t)-12.7(e)0(d)-512.8(b)0(l)-12.2(o)0(o)-7.9(d)-506.8(a)-10.2(n)0(d)-513.8(blo)-16.1(od)-510.7(p)-9.1(rod)-14.4(uc)-8.9(t)-6.7(s)-7.7(,)-506.4(r)-7.7(esu)-16.5(lt)-8.6(in)-11.6(g)-505.8(i)0(n)]tj 0 -1.3858 td 0.0232 tc [(transfusion-transmi)6(tted)-521.4(babesio)6.7(s)-3.4(is)-519.9(\(t)-5.3(tb\))-522(\()]tj 0.30196 0.72157 0.33333 scn 18.7276 0 td 0.0229 tc [(levin)-525.9(a)0.1(nd)-521.7(krause,)]tj -18.7276 -1.3921 td 0 tc (2016)tj 0.15294 0.15294 0.15294 scn 1.915 0 td [(\).)-333.5(in)-335.4(clinical)-338.5(practice,)-336(blood)-331.6(components)-341.5(are)-332.6(often)-337.4(transfused)]tj 26.4504 55.5276 td [(into)-456.8(patients)-467.4(for)-457.6(various)-460.8(purposes:)-465.9(1\))-463(to)-457.8(meet)-466.6(the)-458.1(needs)-464.2(of)-462.5(the)]tj 0 -1.3921 td [(disease,)-343.6(2\))-337.1(to)-344.5(reduce)-340.1(blood)-344.2(transfusion)-341.2(reactions)-343.3(and)-341.4(improve)-348.7(the)]tj 9 0 0 9 304.8944 546.6896 tm [(safety)-251.7(of)-261(blood)-256(transfusion,)-255.4(and)-259.5(3\))-255.2(to)-256.3(facilitate)-257.3(the)-256.5(preservation)-261.1(and)]tj t* [(use)-394.5(of)-393.2(blood)-400.8(components.)-400.7(if)-396.5(blood)-394.5(containing)]tj /f11 1 tf 19.4205 0 td (babesia)tj /f12 1 tf 3.4268 0 td [(is)-396.5(infused)]tj -22.8472 -1.3858 td [(into)-513.5(the)-508.5(hum)-14.2(an)-508.6(bod)-10.7(y,)-508.2(it)-508.9(wi)-7.9(ll)-507.9(caus)-14.2(e)-503.7(vary)-15.3(ing)-511.1(de)-8.9(gre)-9.2(es)-505(of)-512.9(cl)-8(ini)-9.9(cal)]tj 0 -1.3921 td [(reactions)-318.1(depending)-324.5(on)-319.6(the)-319.5(organism)-327(and)-316.2(dosage)-322.3(\()]tj 0.30196 0.72157 0.33333 scn 20.6047 0 td [(eberhard)-324.9(et)-315.1(al.,)]tj -20.6047 -1.3858 td (1995)tj 0.15294 0.15294 0.15294 scn 1.915 0 td [(\).)-478.4(it)-486.3(has)-482.2(been)-482.9(reported)-484.6(that)-481.5(intraperitoneal)-483.6(injection)-488.8(of)-481.4(30)]tj /f11 1 tf -1.915 -1.3921 td (babesia)tj /f12 1 tf 3.4331 0 td [(sporozoites)-407.3(could)-401.9(infect)-407.1(approximately)-410.3(2/5)-401.9(of)-405.8(inoculated)]tj -3.4331 -1.3858 td [(hamsters,)-242.4(while)-247.1(300)-244.9(sporozoites)-249.9(could)-244.4(infect)-249.6(all)-240.9(the)-243.9(inoculated)-247.4(mice)]tj 0 -1.3921 td 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[(infection)-227.3(rate)-225.9(of)-223.2(0.0064\))-225.1(for)-224.5(120)-219.7(days)-224.1(was)-229.1(con)]tj /f13 1 tf 17.8961 0 td ()tj /f12 1 tf 0.5291 0 td [(rmed)-225(to)-224.8(be)]tj /f11 1 tf 4.5039 0 td [(b.)-225.8(microti)]tj /f12 1 tf -22.9291 -1.3858 td [(negative)-340.3(using)-338.9(microscopy)-341.1(and)-335.1(nested)-344.9(pcr)-334.9(test.)-338.1(the)-341.4(whole)-334.6(blood)]tj 0 -1.3921 td 0.0108 tc [(collec)10.8(t)-8.2(e)10.8(d)-514.6(fro)10.8(m)-516.6(t)4.1(h)0.1(ese)-511.8(m)3.9(ice)-505.5(w)1.2(as)-507.1(transfused)-508.6(t)4.1(o)-509.7(si)10.8(x)-514.7(h)0.1(ealt)4.1(hy)-507.6(mic)10.8(e)]tj 0 -1.3858 td 0 tc [(\(second)-414.9(generation\))-417.2(at)-413.8(the)-414(amount)-415.5(of)-412.1(100)-408.7(�l/mouse.)-418(at)-415.7(12)-408.9(and)]tj 0 -1.3921 td 0.0199 tc [(28)-521.6(dpi,)-518(the)-521.6(i)2.7(nf)-4.1(ection)-522.6(s)5.9(tatus)-516.9(o)-2.9(f)-514.3(t)0.6(he)-515.3(m)-5.9(i)2.7(ce)-521.6(was)-516.9(o)-2.9(bserved)-518.4(u)-1.8(sing)]tj 0 -1.3858 td 0 tc [(microscopy)-410.4(\(2.4.1\))-414.2(and)-404.4(the)-407.7(nested)-414.2(pcr)-404.2(test)-411.3(\(2.4.2\))-407.9(to)-407.4(determine)]tj 0 -1.3921 td [(whether)-466.9(there)-471.8(was)-468.4(a)-463.8(risk)-469.4(of)]tj /f11 1 tf 12.2583 0 td [(b.)-465.1(microti)]tj /f12 1 tf 4.5669 0 td [(transmission)-472.1(during)-467.7(the)]tj -16.8252 -1.3858 td [(transfusion)-322.3(of)-311.4(the)-319.5(whole)-309.4(blood)-319(collected)-318.7(from)-313.3(the)-319.5(inoculated)-316.7(mice)]tj 0 -1.3921 td (\()tj /f13 1 tf 0.3465 0 td ()tj /f12 1 tf 0.5291 0 td [(rst)-330.6(generation\).)-337.8(after)-340(0,)-329.8(14,)-335.8(28,)-329.5(and)-335.1(60)-333.3(dpi,)-334(the)-332.1(level)-332.8(changes)-339.3(of)]tj -0.8756 -1.3858 td (speci)tj /f13 1 tf 1.978 0 td ()tj /f12 1 tf 0.5354 0 td [(c)-314.7(antibodies)-322(against)]tj /f11 1 tf 8.2772 0 td [(b.)-320.3(microti)]tj /f12 1 tf 4.2709 0 td [(were)-325.4(quanti)]tj /f13 1 tf 4.7685 0 td ()tj /f12 1 tf 0.5291 0 td [(ed)-323.8(in)-320.3(the)-319.5(serum)]tj -20.3591 -1.3921 td [(using)-301.1(the)-300.6(elisa)-304(test)-304.2(\(2.4.3\).)]tj /f1 1 tf 10.5001 0 0 10.5001 49.6063 232.8945 tm [(2.5.2)-303.1(assessment)-304.6(of)-300.2(human)-304.5(blood)-301(samples)-302.8(using)]tj 0 -1.1446 td [(mouse)-300.3(models)]tj /f12 1 tf 9 0 0 9 63.6094 208.4031 tm [(blood)-273.8(samples)-276.3(\(557\))-270.7(were)-275(used)-277.6(to)-268.9(quantify)-277.8(the)]tj /f11 1 tf 18.8976 0 td [(b.)-269.9(microti)]tj /f12 1 tf 4.1764 0 td (dna)tj -24.6299 -1.3921 td [(using)-351.5(nested)-351.2(pcr)-353.8(\(2.4.2\).)-353.7(of)-349.5(these)-352.1(557)-352(whole)-347.2(blood)-350.5(samples,)-354.4(372)]tj 0 -1.3858 td [(were)-401(used)-403.6(to)-394.8(quantify)-403.8(the)-401.4(speci)]tj /f13 1 tf 13.0457 0 td ()tj /f12 1 tf 0.5291 0 td [(c)-402.9(anti-)]tj /f11 1 tf 2.715 0 td [(b.)-395.8(microti)]tj /f12 1 tf 4.4346 0 td [(antibody)-400.1(using)]tj -20.7244 -1.3921 td [(elisa)-272.5(\(2.4.3\).)-278.1(two)]tj /f11 1 tf 7.9685 0 td [(b.)-276.2(microti)]tj /f12 1 tf 3.9118 0 td [(-positive)-269.9(blood)-274.9(samples)-276.3(\(samples)-273.5(118)]tj -11.8803 -1.3858 td [(and)-265.8(146\))-260.9(were)-262.4(identi)]tj /f13 1 tf 8.2457 0 td ()tj /f12 1 tf 0.5291 0 td [(ed)-267.1(by)-257.7(nested)-269.3(pcr,)-261.8(and)-265.8(six)]tj /f11 1 tf 10.6772 0 td [(b.)-263.6(microti)]tj /f12 1 tf 3.8992 0 td (-positive)tj -23.3512 -1.3921 td [(blood)-438.6(samples,)-442.6(including)-441.8(sample)-438.8(130,)-436.3(were)-438.8(identi)]tj /f13 1 tf 20.6866 0 td ()tj /f12 1 tf 0.5291 0 td [(ed)-437.2(by)-440.4(elisa.)]tj -21.2158 -1.3858 td [(after)-232.9(that,)-238.3(three)-232.4(balb/c)-241.7(and)-234.3(two)-234.4(nod-scid)-235.6(mice)-231.4(were)-237.2(inoculated)]tj 0 -1.3921 td [(with)-384.3(the)-388.8(positive)-384.5(blood)-388.3(of)-386.9(sample)-388.4(118)-383.5(at)-388.6(the)-382.5(amount)-390.3(of)-386.9(100)]tj /f8 1 tf 25.3606 0 td (m)tj /f12 1 tf 0.5732 0 td (l/)tj -25.9339 -1.3858 td [(mouse)-266.7(and)-272.1(were)-268.7(observed)-266.7(for)-268.6(98)-270.3(days.)-270.7(the)-265.8(infection)-271.4(status)-268.5(of)-267.3(these)]tj 0 -1.3921 td [(inoculated)-486.8(mice)-483.4(was)-481(evaluated)-489.6(at)-476.7(dif)56.8(ferent)-485.7(pre-determined)-487.6(time)]tj 0 -1.3858 td [(points)-227.9(using)-231.8(microscopy)-227.7(and)-234.3(nested)-225.2(pcr.)-230.3(at)-226.7(the)-231.3(end)-223.8(of)-229.5(observation)]tj t* [(\(98)-236(days\),)-242.7(the)-243.9(whole)-233.9(blood)-243.4(samples)-238.5(from)-237.7(the)]tj /f13 1 tf 17.8331 0 td ()tj /f12 1 tf 0.5291 0 td [(rst-generation)-245.5(nod-)]tj 10.0031 76.441 td [(scid)-491.1(mice)-496(that)-494.1(tested)]tj /f11 1 tf 9.9591 0 td [(b.)-490.3(microti)]tj /f12 1 tf 4.6173 0 td [(positive)-497.9(were)-495.5(inoculated)-493.1(into)]tj -14.5764 -1.3858 td 0.0302 tc [(h)6.9(e)-1(althy)-526(nod-sc)5.9(i)-2.2(d)-525.8(m)-1.9(ice)-523.9(\()1.5(the)-530.2(s)]tj 14.3496 0 td 0.0286 tc [(econd)-534.9(genera)-6.8(tion\))-529.2(b)-0.5(y)-527.6(b)-0.5(lood)]tj -14.3496 -1.3921 td 0.0134 tc [(tran)-6.2(sfusion)-516.5(a)-3.1(nd)-512.3(the)-515.5(i)2.5(nfec)-5.2(tion)-516.5(stat)-5.9(us)-517.1(of)-514.5(the)-515.5(s)-0.6(e)-5.2(c)1.1(ond-gen)-6.2(e)1.1(ration)]tj 0 -1.3858 td 0 tc [(nod-scid)-361.6(mice)-363.7(was)-361.4(assessed)-364.4(for)-363.1(65)-358.5(days)-362.7(using)-364.1(microscopy)-366.3(and)]tj 0 -1.3921 td [(nested)-288.2(pcr.)-293.3(moreover,)-294.7(to)-287.8(test)-291.6(the)-288(transmission)-295.7(risk)-293.1(of)-286.2(the)-294.3(positive)]tj 0 -1.3858 td [(blood)-438.6(sample130,)-453(we)-444(inoculated)-442.7(three)-446.6(balb/c)-449.6(and)-442.1(three)-446.6(nod-)]tj 0 -1.3921 td 0.0189 tc [(sc)-5.4(id)-518.2(m)-6.9(i)1.7(c)-6(e)-522.6(at)-523.2(t)-6.7(h)1.9(e)-522.6(a)-3.9(mount)-523.2(o)2.4(f)-527.9(100)]tj /f8 1 tf 15.5591 0 td 0 tc (m)tj /f12 1 tf 0.5921 0 td 0.021 tc [(l/mo)4.5(use.)-523.2(at)-521.1(the)-520.5(e)2.4(nd)-523.6(of)]tj -16.1512 -1.3858 td 0 tc [(observation)-397.3(\(98)-399.8(days\),)-400.1(the)-395.1(whole)-397.6(blood)-394.5(of)-399.5(the)]tj /f13 1 tf 19.3953 0 td ()tj /f12 1 tf 0.5354 0 td [(rst)-393.6(generation)-401.2(of)]tj -19.9307 -1.3921 td [(nod-scid)-254.5(mice)-250.3(that)-254.8(tested)-256.4(positive)]tj /f11 1 tf 14.8913 0 td [(b.)-251(microti)]tj /f12 1 tf 4.1386 0 td [(was)-254.3(inoculated)-253.7(into)]tj -19.0299 -1.3858 td 0.0302 tc [(h)6.9(e)-1(althy)-526(nod-sc)5.9(i)-2.2(d)-525.8(m)-1.9(ice)-523.9(\()1.5(the)-530.2(s)]tj 14.3496 0 td 0.0286 tc [(econd)-534.9(genera)-6.8(tion\))-529.2(b)-0.5(y)-527.6(b)-0.5(lood)]tj -14.3496 -1.3921 td 0.0243 tc [(tra)-4.8(n)-1.6(sf)-6(usio)-4.8(n.)-526.2(additi)-5.5(onally,)-526.2(t)-1.3(h)-5.3(e)-523.5(infe)-6.9(ction)-524.5(s)-2.2(ta)-4.8(tus)-525.1(o)-4.8(f)-522.5(t)-1.3(he)-529.8(second)]tj 0 -1.3858 td 0 tc [(generation)-483.1(of)-481.4(nod-scid)-481.3(mice)-483.4(was)-481(assessed)-490.4(for)-482.8(65)-478.2(days)-482.4(using)]tj 0 -1.3921 td [(microscopy)-303.3(and)-297.3(nested)-307.1(pcr.)]tj /f1 1 tf 13.5 0 0 13.5 304.8944 571.4078 tm 0.30196 0.72157 0.33333 scn [(3)-298.9(results)]tj 11.9999 0 0 11.9999 304.8944 546.4062 tm 0.15294 0.15294 0.15294 scn [(3.1)-296.4(infection)-307.9(status)-303.9(of)-300.3(mice)-299(inoculated)-304.9(with)]tj 0 -1.0441 td [(dif)64.2(ferent)-302.5(densities)-306.9(of)]tj /f2 1 tf 9.7324 0 td [(b.)-300.8(microti)]tj /f12 1 tf 9 0 0 9 318.8409 508.8755 tm [(the)-309.9(mice)-300.7(were)-306.5(inoculated)-310.4(with)-302.4(dif)56.8(ferent)-303.1(densities)-311.3(of)]tj /f11 1 tf 21.2976 0 td [(b.)-301.4(microti)]tj /f12 1 tf -22.8472 -1.3858 td [(and)-221.7(observed)-228.9(for)-224.5(270)-226(days.)-232.9(microscopy)-224(examination)-229.1(was)-229.1(performed)]tj 0 -1.3921 td [(on)-300.7(the)-306.9(blood)-300.1(samples)-307.8(collected)-306.1(from)-307(the)-306.9(mice)-307(at)-300.4(dif)56.8(ferent)-303.1(dpi,)-308.8(and)]tj 0 -1.3858 td [(the)-243.9(number)-242.2(of)-248.4(infected)-245.8(mice)-244(in)-244.7(each)-247.2(group)-245.9(at)-243.7(dif)56.8(ferent)-246.4(dpi)-243.3(is)-245.3(shown)]tj 0 -1.3921 td (in)tj 0.30196 0.72157 0.33333 scn 1.0268 0 td [(table)-233.4(1)]tj 0.15294 0.15294 0.15294 scn 2.9291 0 td [(.)-229.2(the)-228(results)-235.7(indicated)-233.5(that)-229.6(increasing)-232.7(the)-231.3(infection)-233.6(rate)-225.9(of)]tj -3.9559 -1.3858 td [(the)-325.8(transfused)-328.2(blood)-325.3(could)-332.6(cause)-328.9(rapid)-330.5(infection)-328.1(of)-324(the)-332.1(inoculated)]tj 0 -1.3921 td [(mice.)-347.3(speci)]tj /f13 1 tf 4.548 0 td ()tj /f12 1 tf 0.5354 0 td [(cally,)-345.8(when)-348.4(the)-344.7(infection)-353.3(rate)-345.6(of)-342.9(the)-351(transfused)-347.1(blood)]tj -5.0835 -1.3858 td [(was)-323.6(20%)-326.6(\(group)-326.9(1\),)-326.9(all)-329.1(inoculated)-329.3(mice)-325.9(were)-325.4(infected)-334(from)-325.9(2)-327.3(dpi,)]tj 0 -1.3921 td [(but)-397.7(no)-401.5(infection)-403.7(was)-405.5(observed)-405.3(in)-402.1(other)-400.4(groups)-404.7(at)-401.2(the)-401.4(same)-408.8(time)]tj 0 -1.3858 td [(point.)-229.1(at)-226.7(5)-232.8(dpi,)-233.2(all)-228.3(mice)-231.4(in)-225.8(groups)-236.6(1)-226.5(and)-228(2)-232.8(\(with)-230.3(an)-231.4(infection)-233.6(rate)-225.9(of)]tj 0 -1.3921 td [(transfused)-246.3(blood)-237.1(of)-242.1(4%\))-242.2(and)-246.9(two)-240.7(out)-240.2(of)-242.1(three)-238.7(mice)-244(\(2/3\))-245(in)-244.7(group)-241.5(3)]tj 0 -1.3858 td [(\(with)-312.2(an)-313.3(infection)-315.5(rate)-314.1(of)-317.7(transfused)-315.6(blood)-319(of)-311.4(0.8%\))-313.7(were)-319.1(infected,)]tj 0 -1.3921 td [(while)-203(those)-211.4(in)-206.9(other)-211.4(groups)-209.4(were)-205.7(not)-212.9(infected.)-210.5(moreover,)-212.8(at)-205.9(7)-207.6(dpi,)-208(all)]tj 0 -1.3858 td [(mice)-439.3(in)-446.2(groups)-444.4(1,)-443.2(2,)-436.9(and)-448.4(3)-440.7(were)-445.1(infected,)-443.6(while)-442.4(those)-444.5(in)-439.9(other)]tj t* [(groups)-215.7(were)-218.3(not.)-221.7(all)-211.4(mice)-218.8(in)-219.5(groups)-217.7(1,)-222.7(2,)-216.4(and)-215.4(3)-220.2(and)-215.4(two)-215.5(out)-221.3(of)-216.9(three)]tj 0 -1.3921 td [(mice)-237.7(\(2/3\))-245(in)-244.7(group)-241.5(4)-239.1(\(with)-242.9(an)-244(infection)-246.2(rate)-244.8(of)-235.8(transfused)-252.6(blood)-237.1(of)]tj 0 -1.3858 td [(0.16%\))-363.8(were)-363.2(infected)-365.5(at)-363.4(8)-358.8(dpi,)-365.5(but)-359.9(those)-368.9(in)-358.1(groups)-368.9(5)-358.8(and)-366.6(6)-358.8(were)]tj 0 -1.3921 td [(negative)-252.1(for)]tj /f11 1 tf 4.9449 0 td [(b.)-251(microti.)]tj /f12 1 tf 4.3654 0 td [(at)-245.6(9)-251.7(dpi,)-252.1(all)-253.5(mice)-250.3(in)-251(groups)-253.5(1-5)-250.2(and)-253.2(two)-247(out)]tj -9.3102 -1.3858 td 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382.337 472.138 0.28345 16.611 re f 382.62 472.138 78.066 0.22678 re f 460.686 472.138 0.22681 16.611 re f 460.913 472.138 84.813 0.22678 re f 49.833 455.527 25.002 0.28348 re f 74.835 455.527 0.22678 16.611 re f 75.061 455.527 76.649 0.28348 re f 151.71 455.527 0.22678 16.611 re f 151.937 455.527 76.649 0.28348 re f 228.586 455.527 0.28348 16.611 re f 228.869 455.527 76.592 0.28348 re f 305.461 455.527 0.28345 16.611 re f 305.745 455.527 76.592 0.28348 re f 382.337 455.527 0.28345 16.611 re f 382.62 455.527 78.066 0.28348 re f 460.686 455.527 0.22681 16.611 re f 460.913 455.527 84.813 0.28348 re f 49.833 438.916 25.002 0.28345 re f 74.835 438.916 m 74.835 455.527 l 75.061 455.527 l 75.061 438.916 l 74.835 438.916 l f* 75.061 438.916 76.649 0.28345 re f 151.71 438.916 m 151.71 455.527 l 151.937 455.527 l 151.937 438.916 l 151.71 438.916 l f* 151.937 438.916 76.649 0.28345 re f 228.586 438.916 m 228.586 455.527 l 228.869 455.527 l 228.869 438.916 l 228.586 438.916 l f* 228.869 438.916 76.592 0.28345 re f 305.461 438.916 m 305.461 455.527 l 305.745 455.527 l 305.745 438.916 l 305.461 438.916 l f* 305.745 438.916 76.592 0.28345 re f 382.337 438.916 m 382.337 455.527 l 382.62 455.527 l 382.62 438.916 l 382.337 438.916 l f* 382.62 438.916 78.066 0.28345 re f 460.686 438.916 m 460.686 455.527 l 460.913 455.527 l 460.913 438.916 l 460.686 438.916 l f* 460.913 438.916 84.813 0.28345 re f 74.835 422.589 0.22678 16.328 re f 151.71 422.589 0.22678 16.328 re f 228.586 422.589 0.28348 16.328 re f 305.461 422.589 0.28345 16.328 re f 382.337 422.589 0.28345 16.328 re f 460.686 422.589 0.22681 16.328 re f bt /f5 1 tf 6.9999 0 0 6.9999 49.6063 760.3652 tm 0.49804 0.49804 0.49412 scn [(table)-306.1(1)-794(detection)-303.3(of)]tj /f10 1 tf 10.7637 0 td [(babesia)-303.4(microti)]tj /f5 1 tf 7.4917 0 td [(infection)-306.8(using)-302.5(giemsa-stained)-306.9(thin)-295.2(blood)-306.3(smears)-303.8(of)-295.5(balb/c)-305.3(mice)-300.4(during)-303.1(a)-295.1(9-month)-308.7(study.)]tj /f1 1 tf 9 0 0 9 219.2881 738.255 tm 1 1 1 scn [(microscopy)-309.7(\(no.)-299.7(positive)-304.5(/)-297.9(no.)-300.9(tested\))]tj -18.2929 -2.5449 td (dpi)tj 4.6047 0.5606 td [(group)-306.5(1)]tj -1.2598 -1.115 td [(\(20%;)-305.6(5)-301(mice\))]tj 9.7638 1.115 td [(group)-300.2(2)]tj -0.926 -1.115 td [(\(4%;)-302.8(3)-301(mice\))]tj 9.4677 1.115 td [(group)-300.2(3)]tj -1.3669 -1.115 td [(\(0.8%;)-303.4(3)-301(mice\))]tj 9.8898 1.115 td [(group)-300.2(4)]tj -1.5811 -1.115 td [(\(0.16%;)-307.8(3)-294.7(mice\))]tj 10.2236 1.115 td [(group)-300.2(5)]tj -1.8394 -1.115 td [(\(0.032%;)-204(3)-206.5(mice\))]tj 10.9606 1.115 td [(group)-306.5(6)]tj -2.2614 -1.115 td [(\(0.0064%;)-299.7(3)-294.7(mice\))]tj /f12 1 tf 6.9999 0 0 6.9999 60.0378 694.0345 tm 0.15294 0.15294 0.15294 scn (0)tj 0 -2.3731 td (2)tj /f16 1 tf 6.2768 0 td ()tj /f12 1 tf 0.5507 0 td (\(5/5\))tj -6.8276 -2.3649 td (3)tj /f16 1 tf 6.2768 0 td ()tj /f12 1 tf 0.5507 0 td (\(5/5\))tj -6.8276 -2.373 td (5)tj /f16 1 tf 6.2768 0 td ()tj /f12 1 tf 0.5507 0 td (\(5/5\))tj /f16 1 tf 10.4317 0 td ()tj /f12 1 tf 0.5507 0 td (\(3/3\))tj /f16 1 tf 10.4317 0 td ()tj /f12 1 tf 0.5507 0 td (\(2/3\))tj -28.7924 -2.373 td (6)tj /f16 1 tf 6.2768 0 td ()tj /f12 1 tf 0.5507 0 td (\(5/5\))tj /f16 1 tf 10.4317 0 td ()tj /f12 1 tf 0.5507 0 td (\(3/3\))tj /f16 1 tf 10.4317 0 td ()tj /f12 1 tf 0.5507 0 td (\(2/3\))tj -28.7924 -2.373 td (7)tj /f16 1 tf 6.2768 0 td ()tj /f12 1 tf 0.5507 0 td (\(5/5\))tj /f16 1 tf 10.4317 0 td ()tj /f12 1 tf 0.5507 0 td (\(3/3\))tj /f16 1 tf 10.4317 0 td ()tj /f12 1 tf 0.5507 0 td (\(3/3\))tj -28.7924 -2.3731 td (8)tj /f16 1 tf 6.2768 0 td ()tj /f12 1 tf 0.5507 0 td (\(5/5\))tj /f16 1 tf 10.4317 0 td ()tj /f12 1 tf 0.5507 0 td (\(3/3\))tj /f16 1 tf 10.4317 0 td ()tj /f12 1 tf 0.5507 0 td (\(3/3\))tj /f16 1 tf 10.4317 0 td ()tj /f12 1 tf 0.5507 0 td (\(2/3\))tj -39.7748 -2.373 td (9)tj /f16 1 tf 6.2768 0 td ()tj /f12 1 tf 0.5507 0 td (\(5/5\))tj /f16 1 tf 10.4317 0 td ()tj /f12 1 tf 0.5507 0 td (\(3/3\))tj /f16 1 tf 10.4317 0 td ()tj /f12 1 tf 0.5507 0 td (\(3/3\))tj /f16 1 tf 10.4317 0 td ()tj /f12 1 tf 0.5507 0 td (\(3/3\))tj /f16 1 tf 10.537 0 td ()tj /f12 1 tf 0.5507 0 td (\(3/3\))tj /f16 1 tf 11.2011 0 td ()tj /f12 1 tf 0.5507 0 td (\(2/3\))tj -62.8493 -2.3649 td (10)tj /f16 1 tf 6.5117 0 td ()tj /f12 1 tf 0.5507 0 td (\(5/5\))tj /f16 1 tf 10.4317 0 td ()tj /f12 1 tf 0.5507 0 td (\(3/3\))tj /f16 1 tf 10.4317 0 td ()tj /f12 1 tf 0.5507 0 td (\(3/3\))tj /f16 1 tf 10.4317 0 td ()tj /f12 1 tf 0.5507 0 td (\(3/3\))tj /f16 1 tf 10.537 0 td ()tj /f12 1 tf 0.5507 0 td (\(3/3\))tj /f16 1 tf 11.2011 0 td ()tj /f12 1 tf 0.5507 0 td (\(3/3\))tj -62.8493 -2.3731 td (14)tj /f16 1 tf 6.5117 0 td ()tj /f12 1 tf 0.5507 0 td (\(5/5\))tj /f16 1 tf 10.4317 0 td ()tj /f12 1 tf 0.5507 0 td (\(3/3\))tj /f16 1 tf 10.4317 0 td ()tj /f12 1 tf 0.5507 0 td (\(3/3\))tj /f16 1 tf 10.4317 0 td ()tj /f12 1 tf 0.5507 0 td (\(3/3\))tj /f16 1 tf 10.537 0 td ()tj /f12 1 tf 0.5507 0 td (\(3/3\))tj /f16 1 tf 11.2011 0 td ()tj /f12 1 tf 0.5507 0 td (\(3/3\))tj -62.8493 -2.373 td (21)tj /f16 1 tf 6.5117 0 td ()tj /f12 1 tf 0.5507 0 td [(\(5/5\))-8360.4(x)-293.3(\(0/3\))]tj /f16 1 tf 21.4141 0 td ()tj /f12 1 tf 0.5507 0 td (\(2/3\))tj /f16 1 tf 10.4317 0 td ()tj /f12 1 tf 0.5507 0 td (\(1/3\))tj /f16 1 tf 10.537 0 td ()tj /f12 1 tf 0.5507 0 td (\(3/3\))tj /f16 1 tf 11.2011 0 td ()tj /f12 1 tf 0.5507 0 td (\(3/3\))tj -62.8493 -2.3731 td (30)tj /f16 1 tf 6.5117 0 td ()tj /f12 1 tf 0.5507 0 td (\(2/5\))tj /f16 1 tf 10.4317 0 td ()tj /f12 1 tf 0.5507 0 td [(\(1/3\))-8360.4(x)-301.4(\(0/3\))-8247.1(x)-301.4(\(0/3\))-8344.2(x)-301.4(\(0/3\))-9024.6(x)-301.4(\(0/3\))]tj -18.0449 -2.373 td [(45)-5440.3(x)-301.4(\(0/5\))-8255.2(x)-293.3(\(0/3\))-8255.2(x)-301.4(\(0/3\))-8247.1(x)-301.4(\(0/3\))-8344.2(x)-301.4(\(0/3\))-9024.6(x)-301.4(\(0/3\))]tj 0 -2.365 td [(60)-5440.3(x)-301.4(\(0/5\))-8255.2(x)-293.3(\(0/3\))-8255.2(x)-301.4(\(0/3\))-8247.1(x)-301.4(\(0/3\))-8344.2(x)-301.4(\(0/3\))-9024.6(x)-301.4(\(0/3\))]tj -0.243 -2.373 td [(120)-5204.3(x)-301.4(\(0/5\))-8255.2(x)-293.3(\(0/3\))-8255.2(x)-301.4(\(0/3\))-8247.1(x)-301.4(\(0/3\))-8344.2(x)-301.4(\(0/3\))-9024.6(x)-301.4(\(0/3\))]tj t* [(150)-5204.3(x)-301.4(\(0/5\))-8255.2(x)-293.3(\(0/3\))-8255.2(x)-301.4(\(0/3\))-8247.1(x)-301.4(\(0/3\))-8344.2(x)-301.4(\(0/3\))-9024.6(x)-301.4(\(0/3\))]tj t* [(270)-5204.3(x)-301.4(\(0/5\))-8255.2(x)-293.3(\(0/2\))-8255.2(x)-301.4(\(0/3\))-8247.1(x)-301.4(\(0/3\))-8344.2(x)-301.4(\(0/3\))-9024.6(x)-301.4(\(0/3\))]tj et 0.63529 0.63529 0.63529 scn 49.606 298.715 m 545.953 298.715 l 545.726 298.431 l 49.833 298.431 l f* 545.953 298.715 m 545.953 70.639 l 545.726 70.923 l 545.726 298.431 l f* 545.953 70.639 m 49.606 70.639 l 49.833 70.923 l 545.726 70.923 l f* 49.606 70.639 m 49.606 298.715 l 49.833 298.431 l 49.833 70.923 l f* q 156.076 285.959 m 439.54 285.959 l 439.54 113.953 l 156.076 113.953 l w n q 283.464508 0 0 172.006241 156.075546 113.952766 cm /im2 do q q bt /f5 1 tf 5.5001 0 0 5.5001 60.8315 101.4236 tm 0.49804 0.49804 0.49412 scn [(figure)-305.5(1)]tj /f4 1 tf 6.9999 0 0 6.9999 60.8315 92.4095 tm [(the)-306.8(infection)-300.4(rate)-300.1(of)-305.1(mice)-299(inoculated)-303.2(with)-299.4(blood)-307(containing)-301.3(dif)63(ferent)-302.7(densities)-300.9(of)]tj /f6 1 tf 36.9482 0 td [(b.)-302.3(microti.)]tj /f4 1 tf 4.9 0 td [(the)-298.7(infection)-308.5(rate)-300.1(of)-297(mice)-307.1(in)-297.6(dif)63(ferent)-302.7(groups)-307.3(was)]tj -41.8482 -1.2878 td [(calculated)-309.6(based)-299.3(on)-301.4(microscopy.)]tj /f1 1 tf -1.6036 102.5107 td [(cai)-304.8(et)-300.8(al.)]tj 57.3986 0 td (10.3389/fcimb.2024.1334426)tj -57.3986 -109.3302 td [(frontiers)-304.6(in)]tj 0.30196 0.72157 0.33333 scn 5.5074 0 td [(cellular)-301.1(and)-302.6(infection)-306(microbiology)]tj 58.8564 0 td (frontiersin.org)tj 0.15294 0.15294 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endstream endobj 55 0 obj<> endobj 56 0 obj<> endobj 57 0 obj<> endobj 58 0 obj<> endobj 59 0 obj<> endobj 60 0 obj<> endobj 61 0 obj<> endobj 62 0 obj<>/border[0 0 0]/type/annot>> endobj 63 0 obj<>/border[0 0 0]/type/annot>> endobj 64 0 obj<>/border[0 0 0]/type/annot>> endobj 65 0 obj<>/colorspace<>/font<>/procset[/pdf/text/imagec/imagei]/extgstate<>>> endobj 66 0 obj<>stream /gs1 gs bt /f12 1 tf 9 0 0 9 49.6063 498.5574 tm /cs6 cs 0.15294 0.15294 0.15294 scn 0 tc 0 tw [(infected)-214.4(in)-213.2(group)-210(2.)-216.4(at)-207.8(the)-212.4(end)-211.2(of)-210.6(the)-212.4(experiment)-215.7(\(270)-210.5(dpi\),)-217.7(all)-209.4(mice)]tj 0 -1.3921 td [(were)-300.2(negative)-302.5(for)]tj /f11 1 tf 7.2252 0 td [(b.)-301.4(microti)]tj /f12 1 tf 4.2331 0 td (infection.)tj /f1 1 tf 11.9999 0 0 11.9999 49.6063 448.5543 tm [(3.2)-303.2(nested)-300.1(pcr)-301.3(detection)-304.1(limit)-302.8(of)]tj /f2 1 tf 0 -1.0441 td [(b.)-300.8(microti)]tj /f12 1 tf 9 0 0 9 63.6094 411.0236 tm [(th)23.7(e)-251.7(n)0(e)18.4(s)0(t)23.5(e)12.9(d)-254.8(p)14(cr)-241.8(de)22.6(te)18.8(c)12.8(t)0(i)20.3(o)0(n)-237.7(l)0(i)18.7(m)0(i)20(t)-246(of)-242.1(th)20.4(e)-245.4(d)0(n)19.2(a)-251.6(an)20.5(d)-248.5(p)0(a)18.4(r)0(a)19.9(s)0(i)25.6(t)0(e)-239.5(d)0(e)16.3(n)0(s)23.1(i)0(t)20.3(y)]tj -1.5559 -1.3858 td (of)tj /f11 1 tf 1.0772 0 td [(b.)-282.5(m)12.5(i)0(c)21.4(r)0(o)19.4(t)0(i)]tj /f12 1 tf 4.1449 0 td [(i)14.3(n)-296.8(wh)23.8(ol)19.3(e)-295.8(b)15(lo)19.3(o)15(d)-298.9(w)15.6(a)0(s)-288.8(d)0(e)22.6(t)0(e)25.1(r)0(m)16.9(i)14.3(ne)18.4(d)-292.6(b)0(y)-282.9(a)0(n)20.5(a)0(l)19.3(y)0(z)23.6(i)0(n)19.9(g)-291.6(11)-289.2(w)15.6(h)0(o)23.1(l)0(e)]tj -5.222 -1.3921 td [(blo)-9.8(od)-517(s)-7.7(ampl)-9.3(es)-523.9(wit)-8.2(h)-514.7(a)0(n)-521.2(e)0(x)-10.5(tre)-7.8(mel)-8.6(y)-518.4(low)]tj /f11 1 tf 16.6866 0 td [(b)-7.5(.)-514.3(mic)-10.2(rot)-12.5(i)]tj /f12 1 tf 4.6929 0 td [(den)-9.6(sity)-524.7(\(pr)-13.9(e-)]tj -21.3795 -1.3858 td 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[(dn)25.5(a)-295.7(i)14.3(n)-296.8(w)15.6(ho)23.1(le)-285.2(b)15(l)0(o)19.3(o)15(d)-292.6(c)0(o)21.5(n)0(t)17.8(a)15(in)19.9(in)19.9(g)]tj /f11 1 tf 13.0961 0 td [(b.)-282.5(m)12.5(i)0(c)21.4(r)0(o)19.4(t)0(i)]tj /f12 1 tf 4.1386 0 td [(de)16.3(ns)23.1(it)20.3(y)]tj -23.9874 -1.3921 td [(vary)-9(ing)-523.7(from)-521.2(2.1)-8.2(4)-510(�)-7.5(10)]tj 6.7499 0 0 5.9992 136.97 290.0409 tm (6)tj 9 0 0 9 144.8504 286.0157 tm [(para)-12.1(sites)-13.7(/)]tj /f8 1 tf 3.811 0 td (m)tj /f12 1 tf 0.5732 0 td [(l)-514.7(\()0(s)-11.1(ampl)-9.3(e)-510(n)0(o)-8.2(.)-512.7(1\))-9.5(,)-512.7(4.2)-8.2(8�10)]tj 6.7499 0 0 5.9992 287.433 290.0409 tm (5)tj 9 0 0 9 49.6063 273.5432 tm [(pa)18.4(ra)26.2(si)19.3(te)18.8(s/)]tj /f8 1 tf 3.6787 0 td (m)tj /f12 1 tf 0.5606 0 td -0.0197 tc [(l)-358(\()-19.7(sa)-19.7(mp)-19.7(le)-359.6(n)-5.1(o)-19.7(.)-347.4(2)-19.7(\),)-356(8)-6.8(.)-19.7(56)-19.7(�1)-6.8(0)]tj 6.7499 0 0 5.9992 182.5511 277.5118 tm 0 tc (4)tj 9 0 0 9 188.7874 273.5432 tm [(pa)18.4(ra)19.9(si)25.6(te)18.8(s/)]tj /f8 1 tf 3.6787 0 td (m)tj /f12 1 tf 0.5669 0 td [(l)-338.3(\()15.4(sa)19.9(mp)21.8(le)-329.3(no)23.3(.)-342.6(3)12.9(\),)]tj -19.7102 -1.3921 td [(1.)-8.5(712)-11.8(�)-7.5(1)0(0)]tj 6.7499 0 0 5.9992 82.885 265.0394 tm (4)tj 9 0 0 9 90.7653 261.0142 tm [(p)-9.1(ara)-9.2(s)-7.7(ite)-11(s)-7.7(/)]tj /f8 1 tf 3.8299 0 td (m)tj /f12 1 tf 0.5795 0 td [(l)-521(\(sa)-8.7(m)-6.9(p)0(l)-11.1(e)-516.3(no)-8.2(.)-519(4)0(\))-9.5(,)-519(3.)-8.5(42)-12.1(4�10)]tj 6.7499 0 0 5.9992 238.3369 265.0394 tm (3)tj 9 0 0 9 246.274 261.0142 tm [(para)-12.1(s)-7.7(ite)-11(s)-7.7(/)]tj /f8 1 tf 3.8299 0 td (m)tj /f12 1 tf 0.5732 0 td (l)tj -26.2551 -1.3858 td [(\(s)20.4(am)20.7(pl)20.4(e)-384(n)14.6(o.)-378.1(5)12.9(\))0(,)-371.3(6)0(.)16.7(8)12.9(4)12.9(8)0(�)17.9(1)12.9(0)]tj 6.7499 0 0 5.9992 141.959 252.5102 tm (2)tj 9 0 0 9 148.6488 248.5417 tm [(pa)18.4(ra)19.9(si)25.6(te)18.8(s/)]tj /f8 1 tf 3.685 0 td (m)tj /f12 1 tf 0.5606 0 td 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[(\).)-365(ho)19.1(we)22.1(ve)23.6(r,)]tj /f11 1 tf 5.0331 0 td [(b.)-364.3(mi)17.9(cr)20.9(o)14.5(t)0(i)]tj /f12 1 tf -22.8535 -1.3921 td [(dn)19.2(a)-257.9(w)0(a)17.9(s)-259.6(no)20.5(t)-258.6(d)0(e)22.6(t)0(e)18.8(c)12.8(ta)20.9(bl)19.3(e)-258(i)0(n)-251(s)0(a)19.9(m)0(p)21.8(l)0(e)23.5(s)-265.9(1)12.9(0)-258(an)20.5(d)-261.1(1)0(1)19.4(,)-260.7(wh)23.8(ic)20.9(h)-262.7(h)0(a)23.1(d)]tj /f11 1 tf 22.9669 0 td [(b.)-251(mi)17.9(cr)20.9(o)14.5(t)0(i)]tj /f12 1 tf -22.9669 -1.3858 td [(de)22.6(ns)16.8(i)14.3(t)0(i)20.3(e)12.8(s)-322.6(o)0(f)-311.4(1)0(.)16.7(0)12.9(9)-321(p)0(a)18.4(r)0(a)26.2(s)0(i)19.3(t)0(e)18.8(s)0(/)]tj /f8 1 tf 10.4063 0 td (m)tj /f12 1 tf 0.5669 0 td [(l)-319.4(a)0(n)20.5(d)-324.1(0)12.9(.)0(2)23(2)-321(pa)18.4(ra)19.9(si)25.6(te)18.8(s/)]tj /f8 1 tf 8.2772 0 td (m)tj /f12 1 tf 0.5606 0 td [(l)14.5(,)-323.7(re)17.8(sp)21(e)12.9(c)12.8(ti)20.3(ve)17.3(ly)21.4(.)-317.4(i)0(n)]tj -19.811 -1.3921 td [(ge)23.6(ne)18.4(ra)19.9(l,)-250.1(th)20.4(e)-264.3(n)0(e)24.7(s)0(t)23.5(e)0(d)-254.5(p)0(c)21.2(r)-261.6(de)22.6(te)18.8(c)12.8(t)0(i)20.3(o)0(n)-250.3(l)0(i)18.7(m)0(i)20(t)0(s)-247.4(f)0(o)22.5(r)-265.9(th)20.4(e)-258(d)0(n)19.2(a)-264.2(a)15(n)0(d)-255.5(d)0(e)22.6(n)0(s)16.8(i)14.3(ty)-254.2(o)15(f)]tj /f11 1 tf 0 -1.3858 td [(b.)-232.1(m)12.5(i)0(c)21.4(r)0(o)19.4(t)0(i)]tj /f12 1 tf 4.0378 0 td [(we)22.1(re)-234.2(3)-239.1(f)0(g)18.3(/)]tj /f8 1 tf 3.8425 0 td (m)tj /f12 1 tf 0.5606 0 td 0.2438 tc [(la)258.8(n)243.8(d)7.2(5)256.7(.)243.8(4)266.8(8)]tj /f11 1 tf 4.3528 0 td 0 tc [(b.)-232.1(m)12.5(i)0(c)21.4(r)0(o)19.4(t)0(i)]tj /f12 1 tf 4.0441 0 td -0.0191 tc [(pa)-19.1(ra)-19.1(si)-19.1(te)-6.3(s)-19.1(/)]tj /f8 1 tf 3.6787 0 td 0 tc (m)tj /f12 1 tf 0.5606 0 td [(l,)-227.4(re)17.8(sp)21(e)12.9(c)0(t)18.8(i)0(v)18.8(e)12.8(ly)21.4(.)]tj /f1 1 tf 11.9999 0 0 11.9999 49.6063 136.0063 tm [(3.3)-303.2(transmission)-304.2(risk)-304.1(assessment)-305.2(of)]tj 0 -1.0394 td [(transfusing)-304.2(blood)-307.1(with)-298.1(extremely)-301(low-)]tj 0 -1.0441 td (density)tj /f2 1 tf 3.5811 0 td [(b.)-300.8(microti)]tj /f1 1 tf 4.7339 0 td [(in)-301.9(mouse)-303.8(models)]tj /f12 1 tf 9 0 0 9 63.6094 86.0031 tm 0.0112 tc [(to)-515.6(assess)-513(the)-511.4(t)-1.8(ra)-5.3(nsmissio)-5.3(n)-512.4(r)3.5(isk)-514(o)1(f)-510.4(t)-1.8(ransf)-6.5(u)2.1(sion)-512.4(blood)-514.5(with)]tj -1.5559 -1.3858 td 0 tc [(extremely)-450.7(low)]tj /f11 1 tf 6.2236 0 td [(b.)-446.2(microti)]tj /f12 1 tf 4.5228 0 td [(density,)-454.8(we)-444(used)-447.7(whole)-448(blood)-444.9(samples)]tj 17.6189 47.2252 td (containing)tj /f11 1 tf 4.4598 0 td [(b.)-200.6(microti)]tj /f12 1 tf 4.0378 0 td [(at)-199.6(a)-205.5(lower)-199.3(density)-206.7(\(1.09)-206.7(parasites/)]tj /f8 1 tf 13.052 0 td (m)tj /f12 1 tf 0.5732 0 td [(l\))-203.2(than)-198.4(that)]tj -22.1228 -1.3921 td [(of)-349.2(the)-351(nested)-357.5(pcr)-353.8(detection)-352.2(limit)-352.2(\(5.48)-351.6(parasites/�l\))-360.7(to)-350.7(inoculate)]tj 0 -1.3858 td [(three)-371(healthy)-382.1(balb/c)-374(mice.)-378.8(the)-372.8(blood)-375.6(samples)-383.4(of)-374.4(the)-376.2(inoculated)]tj 0 -1.3921 td [(mice)-477.1(were)-476.6(collected)-482.5(28)-478.2(days)-482.4(after)-476.6(inoculation)-480.8(and)-479.9(analyzed)-480.9(via)]tj 0 -1.3858 td [(nested)-263(pcr)-259.3(\()]tj 0.30196 0.72157 0.33333 scn 5.2598 0 td [(figure)-265.1(4a)]tj 0.15294 0.15294 0.15294 scn 3.9685 0 td [(\).)-264.2(at)-264.5(14)-257.7(and)-265.8(28)-264(dpi,)-264.7(only)-260.4(one)-262.7(mouse)-266.7(\(no.)-259.8(2\))]tj -9.2283 -1.3921 td [(was)-273.2(infected)-277.3(with)]tj /f11 1 tf 7.2315 0 td [(b.)-276.2(microti)]tj /f12 1 tf 3.9118 0 td [(.)-273.3(the)-278.4(level)-269.8(of)-279.9(anti-)]tj /f11 1 tf 7.3575 0 td [(b.)-269.9(microti)]tj /f12 1 tf 4.1764 0 td (antibodies)tj -22.6772 -1.3858 td [(in)-269.9(the)-275.4(blood)-274.9(collected)-274.6(from)-275.5(the)-269.1(inoculated)-278.9(mice)-275.5(were)-275(detected)-276.2(with)]tj 0 -1.3921 td [(elisa)-404.8(at)-413.8(dif)63.1(ferent)-410.1(time)-414.7(points)-410.6(during)-411(the)-407.7(28)-408.9(days.)-415.6(as)-410.4(shown)-410.6(in)]tj 0.30196 0.72157 0.33333 scn 0 -1.3858 td 0.0113 tc [(fi)11.3(gure)-505(4)11.3(b)]tj 0.15294 0.15294 0.15294 scn 4.1764 0 td 0.0089 tc [(,)-510.1(a)-1.3(mon)-4.4(g)-503.2(t)-4.1(h)8.9(e)-518.1(t)2.2(hre)-3.4(e)-507.4(mic)8.9(e)-9.5(,)-510.1(o)-1.3(ne)-507.4(m)-4.3(o)8.9(u)-4.2(s)-5.1(e)-507.4(\()-0.9(no)8.9(.)-514(2)-3.4(\))-504.8(a)-1.4(lways)]tj -4.1764 -1.3921 td 0 tc [(produced)-299.6(the)-306.9(highest)-300.2(amount)-302.1(of)-305.1(antibodies)-303.1(against)]tj /f11 1 tf 20.8063 0 td [(b.)-307.7(microti)]tj /f12 1 tf 4.2394 0 td [(at)-300.4(7,)]tj -25.0457 -1.3858 td [(14,)-298(and)-303.6(28)-301.8(dpi.)]tj 1.5496 -1.3921 td [(according)-480.5(to)-470.4(the)-477(nested)-470.9(pcr)-473.5(and)-473.6(microscopic)-482.2(examination)]tj -1.5496 -1.3858 td [(used)-315.4(to)-313(determine)-324.6(the)-313.2(infection)-321.8(status)-318.9(of)-311.4(the)-319.5(mice)-313.3(inoculated)-323(with)]tj 0 -1.3921 td [(dif)63.1(ferent)-347.1(densities)-349.1(of)]tj /f11 1 tf 8.6173 0 td [(b.)-345.5(microti)]tj /f12 1 tf 4.3276 0 td [(\(3.1\),)-345.2(all)-348(the)-344.7(mice)-351.1(inoculated)-348.2(with)]tj -12.9449 -1.3858 td [(whole)-208.7(blood)-218.2(at)-212.2(the)-212.4(lowest)-218.5(infection)-214.7(rate)-213.3(\(group)-219.8(6\))-211.1(for)-218.2(120)-213.4(days)-211.5(were)]tj 0 -1.3921 td [(not)-364.1(infected)-371.8(with)]tj /f11 1 tf 7.3764 0 td [(b.)-370.6(microti)]tj /f12 1 tf 4 0 td [(.)-367.8(t)0(o)-366.3(evaluate)-373.4(the)-363.6(transmission)-371.3(risk)-368.7(of)]tj -11.3764 -1.3858 td [(transfusing)-370.6(the)-363.6(blood)-363.1(of)-368(these)-371(mice)-363.7(to)-363.3(healthy)-369.5(mice,)-366.2(we)-368.4(collected)]tj 0 -1.3921 td [(and)-202.8(transfused)-208.5(their)-199.4(blood)-205.6(into)-198.6(six)-205.6(healthy)-205.7(balb/c)-204(mice.)-202.4(during)-208.3(the)]tj 0 -1.3858 td [(28)-264(days)-274.5(of)-267.3(observation,)-273.8(no)]tj /f11 1 tf 10.778 0 td [(b.)-269.9(microti)]tj /f12 1 tf 4.1764 0 td [(was)-266.9(found)-273.6(in)-269.9(the)-269.1(blood)-268.6(of)-273.6(the)]tj -14.9543 -1.3921 td [(inoculated)-222.2(mice)-225.1(via)-218.2(microscopic)-230.2(examination;)-225.3(however,)-224.7(nested)-225.2(pcr)]tj 0 -1.3858 td [(results)-273.5(showed)-273.9(that)-267.4(2)-276.9(o)0(f)-267.3(the)-275.4(6)-270.6(mice)-275.5(were)-268.7(infected)-277.3(with)]tj /f11 1 tf 21.8646 0 td [(b.)-276.2(microti)]tj /f12 1 tf 4.1827 0 td (at)tj -26.0473 -1.3921 td [(12)-282.9(and)-291(28)-289.2(dpi)-287.4(\()]tj 0.30196 0.72157 0.33333 scn 6.2047 0 td [(figure)-290.3(4c)]tj 0.15294 0.15294 0.15294 scn 3.9685 0 td [(\).)-289.4(the)-284.7(elisa)-291.4(was)-285.8(used)-290.2(to)-287.8(test)-285.3(the)-288(level)-288.7(of)]tj -10.1732 -1.3858 td [(the)-382.5(speci)]tj /f13 1 tf 3.6095 0 td ()tj /f12 1 tf 0.5354 0 td [(c)-384(anti-)]tj /f11 1 tf 2.6961 0 td [(b.)-383.2(microti)]tj /f12 1 tf 4.4031 0 td [(antibody)-387.5(after)-388.4(60)-383.7(days)-387.9(of)-387(inoculation,)]tj -11.2441 -1.3921 td [(and)-461(the)-464.4(results)-468.8(showed)-462.8(that)-462.6(the)-464.4(antibody)-469.4(levels)-466.5(were)-464(quite)-461.3(low)]tj 0 -1.3858 td [(throughout)-297.2(the)-306.9(60-day)-296.6(observation)-309.1(period)-298.7(\()]tj 0.30196 0.72157 0.33333 scn 17.5811 0 td [(figure)-302.9(4d)]tj 0.15294 0.15294 0.15294 scn 4.0504 0 td (\).)tj /f1 1 tf 11.9999 0 0 11.9999 304.8944 161.0078 tm [(3.4)-299(transmission)-308.9(risk)-299.3(assessment)-305.2(of)]tj 0 -1.0394 td [(transfusing)-304.2(human)-305.3(blood)-302.4(in)-301.9(mouse)-299.1(models)]tj /f12 1 tf 9 0 0 9 318.8409 123.5339 tm [(after)-295.9(the)-294.3(human)-296(blood)-293.8(infected)-296.2(with)]tj /f11 1 tf 15.1181 0 td [(b.)-295.1(microti)]tj /f12 1 tf 4.2205 0 td [(was)-292.1(transfused)]tj -20.8882 -1.3921 td [(to)-426.3(healthy)-426.2(mice,)-429.2(the)-426.6(infection)-435.2(status)-425.9(of)-424.7(the)-432.9(inoculated)-430.1(mice)-426.7(was)]tj 0 -1.3858 td [(determined)-321.1(using)-313.7(nested)-319.7(pcr)-316(and)-316.2(elisa.)-319.1(among)-321.7(the)-313.2(557)-320.5(human)]tj 0 -1.3921 td 0.022 tc [(wh)5(ole)-525.8(b)-0.8(lood)-522.6(samples,)-528.5(two)]tj /f11 1 tf 12.063 0 td 0.0211 tc [(b.)-524.7(m)-4.2(i)1.3(cr)-5.5(oti)]tj /f12 1 tf 4.3591 0 td 0.0222 tc [(-)4.5(p)-5.8(ositive)-525.6(b)-0.6(lood)-522.4(samples)]tj -16.4221 -1.3858 td 0 tc [(\(samples)-437.3(no.)-439(118)-440.2(and)-442.1(146\))-437.3(were)-438.8(identi)]tj /f13 1 tf 16.4913 0 td ()tj /f12 1 tf 0.5291 0 td [(ed)-443.5(by)-434.1(nested)-445.7(pcr,)-438.2(and)]tj et 0.63529 0.63529 0.63529 scn 49.606 765.524 m 545.953 765.524 l 545.726 765.297 l 49.833 765.297 l f* 545.953 765.524 m 545.953 522.709 l 545.726 522.935 l 545.726 765.297 l f* 545.953 522.709 m 49.606 522.709 l 49.833 522.935 l 545.726 522.935 l f* 49.606 522.709 m 49.606 765.524 l 49.833 765.297 l 49.833 522.935 l f* q 156.076 752.825 m 439.54 752.825 l 439.54 565.965 l 156.076 565.965 l w n q 283.464508 0 0 186.859802 156.075546 565.965271 cm /im3 do q q bt /f5 1 tf 5.5001 0 0 5.5001 60.8315 553.4361 tm 0.49804 0.49804 0.49412 scn [(figure)-305.5(2)]tj /f4 1 tf 6.9999 0 0 6.9999 60.8315 544.4219 tm [(the)-306.8(antibody)-303.9(levels)-296.3(in)-305.7(mice)-299(inoculated)-303.2(with)-299.4(blood)-307(containing)-301.3(dif)63(ferent)-302.7(densities)-300.9(of)]tj /f6 1 tf 37.5314 0 td [(babesia)-300.2(microti.)]tj /f4 1 tf 7.5079 0 td [(the)-298.7(levels)-304.4(of)-297(speci)]tj /f7 1 tf 8.3907 0 td ()tj /f4 1 tf 0.5345 0 td [(c)-301.3(antibodies)-303.8(against)]tj /f6 1 tf 9.2816 0 td [(b.)-294.2(microti)]tj /f4 1 tf -63.2461 -1.2797 td [(produced)-304.6(in)-305.7(mice)-299(at)-299(dif)63(ferent)-302.7(time)-304.5(points)-298.7(in)-305.7(dif)63(ferent)-302.7(groups)-299.2(were)-302.3(detected)-304.1(by)-296.8(enzyme-linked)-305.6(immunosorbent)-304(assay)-306(\(elisa\).)]tj /f1 1 tf -1.6036 37.9282 td [(cai)-304.8(et)-300.8(al.)]tj 57.3986 0 td (10.3389/fcimb.2024.1334426)tj -57.3986 -109.3302 td [(frontiers)-304.6(in)]tj 0.30196 0.72157 0.33333 scn 5.5074 0 td [(cellular)-301.1(and)-302.6(infection)-306(microbiology)]tj 58.8564 0 td (frontiersin.org)tj 0.15294 0.15294 0.15294 scn -29.5213 0 td (06)tj et endstream endobj 67 0 obj<>stream h��mk�`����~��l#��-�v �v�r�3q��(a�좇��&̮���l-��v���x��s{ �f#le���j�r���:p�kf��"��} �r��j��q�2�8�7bn�^-�n�e@t�6̪�t��lԓ���s.zd��f)��i�9y@���\z@�ڇ���0����k� ��w�?�5w]!� |8�m�ҏ�v����h~.h��)�i�q9-��bp�rn���-�kf���oe���c8��z��"f�ġz���n���y����z����w�ע@`)���y {��� 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